Elisa kits have revolutionized biomedical research, clinical diagnostics, and drug development due to their high sensitivity, specificity, and ease of use. This article provides an overview of the principles underlying Elisa assays and explores their diverse applications in different fields.

What is Elisa?

Definition and historical background

Key components of an Elisa kit: microplate, antibodies, enzymes, and substrates

Principles of Elisa Assays:

Immunoassay principles: antigen-antibody interactions

Types of Elisa assays: direct, indirect, sandwich, and competitive

Signal amplification mechanisms: enzyme-substrate reactions

Workflow of an Elisa Assay:

Step-by-step description of the assay procedure

Sample preparation and plate coating

Incubation, washing, and detection steps

Data analysis and interpretation.

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